FACILITIES AND RESOURCES
Duke One Health Research Laboratory (OHRL):The Duke One Health Research Laboratory (OHRL) is directed by Dr. Greg Gray and focuses on research and training related to emerging infectious diseases, particularly those that are zoonotic. The OHRL is capable of viral and bacterial culture, molecular identification, genotyping, serological studies, and laboratory assay development. The OHRL has a reputation for excellence in respiratory virus work, and has previously conducted complex prospective cohort epidemiological studies involving more than 3000 persons living in 8 countries (Australia, Cambodia, Mexico, Mongolia, Nigeria, Romania, Thailand, United States), for their infection with emerging pathogens. The OHRL is currently conducting studies of influenza A, B, D, and D viruses, adenoviruses, coronaviruses, and enteroviruses but in the recent past has performed studies of all of the pathogens summarized in Table 1. The OHRL has conducted recent laboratory analyses in BSL2+Ag and BSL3 laboratories and has a large collection of human and animal respiratory viruses.
|Table 1. Summary of pathogens that can be studied at the Duke One Health Research Laboratory.|
|Human adenovirus||Swine influenza virus|
|Various animal adenoviruses||Avian influenza virus|
|Human influenza virus||Equine influenza virus|
|Canine influenza virus||Rift Valley fever virus|
|Human respiratory syncytial viruses||Bovine respiratory syncytial virus|
|Bovine rotavirus||Human parainfluenza viruses|
|Human coronaviruses||Caprine arthritis encephalitis virus|
|Canine respiratory coronavirus||Human rhinoviruses|
|Human metapneumovirus||Human enteroviruses|
|Turkey pneumoviruses||Human bocavirus|
|Influenza B||Caprine anemia encephalitis virus|
|Porcine reproductive respiratory disease syndrome virus (PRRSV)|
The OHRL is located in Alexander Sands building, housed within the Duke University Medical complex. Major equipment available in this facility include:, 2 class II A1 biological safety cabinets, 2 humidified incubators with CO2 supply for uninfected tissue culture work and virus propagation/isolation, microcentrifuges, a tabletop refrigerated centrifuge, a Metler analytical balance, an Acculab digital balance, two inverted microscopes, two BioRad sub-cell GT agarose gel electrophoresis platforms, a BioRad Protean II SDS-PAGE and western blot platform, a microarray scanner, BioRad iCycler with iQ5 real-time PCR platform, BioRad MyCycler PCR thermal cycler, Life Technologies 7500dx real-time PCR platform, Thermo KingFisher nucleic acid purification instrument, BioTek EL406 plate washer with 50 plate capacity BioTek plate stacker and BioTek PowerWave 340 microplate-sprectophotometer with an attached 50 plate capacity BioTek plate stacker, a fluorescence capable microtiter plate reader, a lab-dishwasher, two autoclaves, a Millipore water purification system, an ice machine, a U/V fluorescent microscope, shaking incubators, a walk-in refrigerator, a AKTA-FPLC, 1 20/4°C freezer refrigerator, 1 -20°C freezer, 2 -80°C freezers, 6 desk top computers and 2 printers, with access to a shared network printer/scanner/fax.
Duke-NUS Laboratory of One Health Research: The Laboratory of One Health Research at Duke-NUS Medical School Singapore belongs to a multisite One Health research network lead by Dr Gregory C. Gray. As an important expansion of Duke One Health team in the USA, the Laboratory of One Health Research was established in August 2014 under Program in Emerging Infectious Diseases (EID) at Duke-NUS Medical School, Singapore with a goal of leading interdisciplinary One Health research and training activities in Singapore and other countries across South Asia region. Our laboratory in Singapore currently focuses on detecting emerging and/or zoonotic respiratory viruses (e.g. adenoviruses, influenza A and B ,coronaviruses) in clinical settings, among military populations, and at the human-animal interface using various well-established techniques (e.g. molecular typing, virus isolation in BSL2 facilities, whole genome sequencing, serology tests and bioaerosol sampling methods) as well as conducting One Health training (e.g. one health approach, epidemiology, molecular diagnostics, and virology) for public health and veterinarian professionals from developing countries.
|Table 2. Summary of pathogens that can be studied at the Duke-NUS Laboratory of One Health Research.|
|Human & animal adenoviruses|
|Human & animal influenza viruses|
|Human & animal coronaviruses|
|Human & animal enteroviruses|
The Laboratory of One Health Research is housed within Duke-NUS Graduate Medical School that is situated in the heart of the country’s largest healthcare cluster, Singapore Healthcare Group (SingHealth). Major equipment available in the Laboratory of One Health Research include: 2 class II A1 biological safety cabinets, 2 humidified incubators with CO2 supply for uninfected tissue culture work and virus propagation/isolation, microcentrifuges, a tabletop refrigerated centrifuge, 2 BioRad sub-cell GT agarose gel electrophoresis platforms, a Biorad T100 thermal cycler, a C1000 thermal cycler, a Grant bio UVT-B-AR PCR cabinet, a 4°C freezer refrigerator, a -20°C freezer, a -80°C freezer, 2 SKC BioSampler units (glassware impinger) and 2 PTFE filter-based air sampler units. Other core equipment are accessible via shared facilities in Duke-NUS such as Biorad CFX96 real-time PCR machine, Tecan Infinite M200 microplate reader, ImageQuant-RT ECL machine, Nanodrop machine, gel-doc machine, confocal microscopes, fluorescent microscope, autoclave machines, 2 desk top computers and 1 printer, with access to a shared network printer/scanner/fax, and glassware washing equipment.
Duke Kunshan University: Duke Kunshan University (DKU) is a new, comprehensive, international Chinese university co-founded by Duke University, Wuhan University, and Kunshan municipal government. The overall construction budget is expected to reach five billion RMB for the construction of the entire campus of 850k m2 (approx. 210.8 acres) and 45k m2 floor areas. At this moment, 1.3 billion RMB has been invested to complete the phase I construction of 68k m2 floor areas.
Duke Kunshan University is establishing a series of interdisciplinary research centers as platforms for undergraduate, graduate and postdoctoral education. These Centers are tackling real world research problems. One such center is the Global Health Research Center. Five DKU full-time faculty, two DKU part-time faculty, three post-doctoral researchers, five research assistants and about 20 collaborators from Wuhan University, local Chinese Centers for Disease Control and Prevention, and other Chinese universities are conducting research together through this center. Currently their research focuses upon public health, law, environment science, social sciences and infectious diseases. DKU is developing a number of additional research centers that will conduct research in the areas of environment management and policies, physical science and engineering, and studies on higher education.
DKU’s current facility provides office space for faculty and research fellows, library and online research database access, and information technology support for research data storage, backup and security.
An Innovation Building is under construction that will soon provide researchers with 5270 square feet of additional office space, 2700 square feet of dry laboratory space and 4800 square feet of wet laboratory space. The new laboratories will be equipped with a sample processing rooms, a sample purification room, a cell culture room, thermocyclers, refrigerated and ultracold storage, and water purification systems.
Dr. Gray and Dr. Wang are setting up a temporary molecular diagnostic laboratory for respiratory viruses in the convention center that later will transition into a BSL2 viral laboratory in the new Innovation Building. Currently research involves studies of adenoviruses and influenza viruses.